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cd137 microbead kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd137 microbead kit
    Cd137 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd137+microbead+kit/CD137+MicroBead+Kit%2C+human/10__1002_slash_viw__20250212-80-24-27
    Average 94 stars, based on 34 article reviews
    cd137 microbead kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Enhancement of immune checkpoint inhibitor efficacy by DHX58+ SELL+ T cells in advanced gastric cancer
    Article Snippet: .. For expansion of tumor-reactive sublines, T cells were sorted on the basis of CD137 expression 24 h after stimulation with tumor organoids, using a CD137 Microbead Kit (Miltenyi) followingmanufacturer’s instructions and expanded as described above. ..

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient.
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137+ cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137+/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO2 until functional assessment.

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137 + cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137 +/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO 2 until functional assessment.

    Isolation:

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient.
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137+ cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137+/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO2 until functional assessment.

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137 + cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137 +/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO 2 until functional assessment.

    FACS:

    Article Title: Dupilumab strengthens herpes simplex virus type 1-specific immune responses in atopic dermatitis.
    Article Snippet: 1 rom the Department of Dermatology and Allergy, and the Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover. he first 2 authors contributed equally to this article, and both should be considered first author. eceived for publication December 16, 2022; revised June 22, 2023; accepted for publication August 3, 2023. vailable online September 1, 2023. orresponding author: Stephan Traidl, MD, Department of Dermatology and Allergy, Division of Immunodermatology and Allergy Research, Hannover Medical School (MHH), Carl-Neuberg-Str 1, 30625 Hannover, Germany.. E-mail: traidl.stephan@ mh-hannover.de.. The CrossMark symbol notifies online readers when updates have been made to the article such as errata or minor corrections 091-6749/$36.0

    Labeling:

    Article Title: High-resolution profile of neoantigen-specific TCR activation links moderate stimulation to increased resilience of engineered TCR-T cells
    Article Snippet: .. Another 24 h later, reactive T cells were separated using magnetic labeling and positive selection with the CD137 MicroBead Kit (Miltenyi, cat. 130-093-476). ..

    Article Title: Prolonged response after TPO-RA discontinuation in primary ITP: results of a prospective multicenter study.
    Article Snippet: We adapted the assay from the originally described antigenreactive T-cell enrichment procedure17 and activation-induced marker assay.18,19 In brief, ~0.5 × 107 to 4 × 107 cryopreserved PBMCs were plated in RPMI 1640 medium (Gibco), supplemented with 5% human AB serum (Sigma Aldrich, Schnelldorf, Germany), and stimulated with GPIIb/IIIa peptides or dimethyl sulfoxide alone for 24 hours in the presence of 1 mg/mL CD40 and 1 mg/ mL CD28 pure antibody (both from Miltenyi Biotec, Bergisch Gladbach, Germany). .. Cells were labeled with CD154-biotin and CD137-PE, and anti-biotin (CD154 MicroBead Kit, Miltenyi Biotec) and anti-PE (CD137 MicroBead Kit, Miltenyi Biotec) antibodies were added, then were magnetically enriched by using MS Columns (Miltenyi Biotec). ..

    Selection:

    Article Title: High-resolution profile of neoantigen-specific TCR activation links moderate stimulation to increased resilience of engineered TCR-T cells
    Article Snippet: .. Another 24 h later, reactive T cells were separated using magnetic labeling and positive selection with the CD137 MicroBead Kit (Miltenyi, cat. 130-093-476). ..



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    Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of CD4 + CD154 + Th cells in sepsis patients after stimulation with control or indicated antigens ( n = 20). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of CD4 + CD154 + Th cells in sepsis patients after stimulation with control or indicated antigens ( n = 20). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Control

    Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of IFNγ producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of TNF-α producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of IL-2 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of IFNγ producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of TNF-α producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of IL-2 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques:

    Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 2 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 10/10/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 2 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 10/10/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Control

    Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 2. ( A ) Frequencies of IL-17 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( B ) Frequencies of IL-4 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( C ) Frequencies of IL-10 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 2. ( A ) Frequencies of IL-17 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( B ) Frequencies of IL-4 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( C ) Frequencies of IL-10 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques:

    Bifidobacterium longum -reactive gut trophic Th cell signature ( A ) Frequencies of CD4 + CD154 + α4ß7 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001. ( B ) Frequencies of B. longum reactive Th cells in cohort 1 and 2. ( C ) Frequencies of IFNγ- and TNF-α producing B. longum reactive Th cells in cohort 1 and 2. ( D ) Frequencies of gut trophic α4ß7 + -expressing B. longum- reactive Th cells. (B) and (C) Merged data from both cohorts. Color code indicating respective groups

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Bifidobacterium longum -reactive gut trophic Th cell signature ( A ) Frequencies of CD4 + CD154 + α4ß7 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001. ( B ) Frequencies of B. longum reactive Th cells in cohort 1 and 2. ( C ) Frequencies of IFNγ- and TNF-α producing B. longum reactive Th cells in cohort 1 and 2. ( D ) Frequencies of gut trophic α4ß7 + -expressing B. longum- reactive Th cells. (B) and (C) Merged data from both cohorts. Color code indicating respective groups

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Expressing